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Australian bat lyssavirus infection in two horses

Shinwari, Mustaghfira Wafa and Annand, Edward J. and Driver, Luke and Warrilow, David and Harrower, Bruce and Allcock, Richard J. N. and Pukallus, Dennis and Harper, Jennifer and Bingham, John and Kung, Nina and Diallo, Ibrahim S. (2014) Australian bat lyssavirus infection in two horses. Veterinary Microbiology, 173 (3–4). pp. 224-231. ISSN 0378-1135

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Article Link(s): http://dx.doi.org/10.1016/j.vetmic.2014.07.029

Publisher URL: http://www.sciencedirect.com/science/article/pii/S037811351400371X

Abstract

In May 2013, the first cases of Australian bat lyssavirus infections in domestic animals were identified in Australia. Two horses (filly-H1 and gelding-H2) were infected with the Yellow-bellied sheathtail bat (YBST) variant of Australian bat lyssavirus (ABLV). The horses presented with neurological signs, pyrexia and progressing ataxia. Intra-cytoplasmic inclusion bodies (Negri bodies) were detected in some Purkinje neurons in haematoxylin and eosin (H&E) stained sections from the brain of one of the two infected horses (H2) by histological examination. A morphological diagnosis of sub-acute moderate non-suppurative, predominantly angiocentric, meningo-encephalomyelitis of viral aetiology was made. The presumptive diagnosis of ABLV infection was confirmed by the positive testing of the affected brain tissue from (H2) in a range of laboratory tests including fluorescent antibody test (FAT) and real-time PCR targeting the nucleocapsid (N) gene. Retrospective testing of the oral swab from (H1) in the real-time PCR also returned a positive result. The FAT and immunohistochemistry (IHC) revealed an abundance of ABLV antigen throughout the examined brain sections. ABLV was isolated from the brain (H2) and oral swab/saliva (H1) in the neuroblastoma cell line (MNA). Alignment of the genome sequence revealed a 97.7% identity with the YBST ABLV strain.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Australian bat lyssavirus Equine non-suppurative meningoencephalomyelitis Negri bodies Yellow-bellied sheathtail bat
Subjects:Veterinary medicine > Diseases of special classes of animals > Horses
Veterinary medicine > Veterinary virology
Deposited On:21 Jan 2015 03:56
Last Modified:08 Jun 2015 16:03

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