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Grain sorghum proteomics: integrated approach toward characterization of endosperm storage proteins in kafirin allelic variants

Cremer, J. E. and Bean, S. R. and Tilley, M. M. and Ioerger, B. P. and Ohm, J. B. and Kaufman, R. C. and Wilson, J. D. and Innes, D. J. and Gilding, E. K. and Godwin, I. D. (2014) Grain sorghum proteomics: integrated approach toward characterization of endosperm storage proteins in kafirin allelic variants. J Agric Food Chem, 62 (40). pp. 9819-31. ISSN 1520-5118 (Electronic)0021-8561 (Linking)

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Article Link(s): http://dx.doi.org/10.1021/jf5022847

Abstract

Grain protein composition determines quality traits, such as value for food, feedstock, and biomaterials uses. The major storage proteins in sorghum are the prolamins, known as kafirins. Located primarily on the periphery of the protein bodies surrounding starch, cysteine-rich beta- and gamma-kafirins may limit enzymatic access to internally positioned alpha-kafirins and starch. An integrated approach was used to characterize sorghum with allelic variation at the kafirin loci to determine the effects of this genetic diversity on protein expression. Reversed-phase high performance liquid chromatography and lab-on-a-chip analysis showed reductions in alcohol-soluble protein in beta-kafirin null lines. Gel-based separation and liquid chromatography-tandem mass spectrometry identified a range of redox active proteins affecting storage protein biochemistry. Thioredoxin, involved in the processing of proteins at germination, has reported impacts on grain digestibility and was differentially expressed across genotypes. Thus, redox states of endosperm proteins, of which kafirins are a subset, could affect quality traits in addition to the expression of proteins.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Cremer, Julia E Bean, Scott R Tilley, Michael M Ioerger, Brian P Ohm, Jae B Kaufman, Rhett C Wilson, Jeff D Innes, David J Gilding, Edward K Godwin, Ian D Research Support, Non-U.S. Gov't United States J Agric Food Chem. 2014 Oct 8;62(40):9819-31. doi: 10.1021/jf5022847. Epub 2014 Sep 24.
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Sorghum
Deposited On:21 Jan 2015 04:02
Last Modified:03 Feb 2015 01:58

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