Walczyk, N. E. and Smith, P. M. C. and Tovey, E. and Wright, G. C. and Fleischfresser, D. B. and Roberts, T. H. (2013) Analysis of Crude Protein and Allergen Abundance in Peanuts (Arachis hypogaea cv. Walter) from Three Growing Regions in Australia. Journal of Agricultural and Food Chemistry, 61 (15). pp. 3714-3725. ISSN 0021-8561
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Article Link(s): http://dx.doi.org/10.1021/jf305347r
The effects of plant growth conditions on concentrations of proteins, including allergens, in peanut (Arachis hypogaea L.) kernels are largely unknown. Peanuts (cv. Walter) were grown at five sites (Taabinga, Redvale, Childers, Bundaberg, and Kairi) covering three commercial growing regions in Queensland, Australia. Differences in temperature, rainfall, and solar radiation during the growing season were evaluated. Kernel yield varied from 2.3 t/ha (Kairi) to 3.9 t/ha (Childers), probably due to differences in solar radiation. Crude protein appeared to vary only between Kairi and Childers, whereas Ara h 1 and 2 concentrations were similar in all locations. 2D-DIGE revealed significant differences in spot volumes for only two minor protein spots from peanuts grown in the five locations. Western blotting using peanut-allergic serum revealed no qualitative differences in recognition of antigens. It was concluded that peanuts grown in different growing regions in Queensland, Australia, had similar protein compositions and therefore were unlikely to show differences in allergenicity.
|Business groups:||Crop and Food Science|
|Keywords:||peanut Arachis hypogaea allergen allergenicity Ara h 1 Ara h 2 2D-DIGE growth conditions ara h 1 ige-binding epitopes major allergen short episodes heat-stress seed ara-h-2 pollen yield l.|
|Subjects:||Plant culture > Fruit and fruit culture > Nuts|
|Deposited On:||28 Aug 2014 03:29|
|Last Modified:||28 Aug 2014 03:29|
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