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Mapping of genomic regions associated with net form of netblotch resistance in barley

Raman, H. and Platz, G.J. and Chalmers, K.J. and Raman, R. and Read, B.J. and Barr, A.R. and Moody, D.B. (2003) Mapping of genomic regions associated with net form of netblotch resistance in barley. Australian Journal of Agricultural Research, 54 (12). pp. 1359-1367.

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Article Link(s): http://dx.doi.org/10.1071/AR03026

Publisher URL: http://www.publish.csiro.au

Abstract

Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9–17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population.

In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Pyrenophora teres f. teres; QTL; seedling resistance; validation.
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Plant pests and diseases > Individual or types of plants or trees > Barley
Deposited On:08 Jun 2004
Last Modified:08 Jun 2015 15:50

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