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Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense

Fraser-Smith, S. and Czislowski, E. and Meldrum, R. A. and Zander, M. and O'Neill, W. and Balali, G. R. and Aitken, E. A. B. (2014) Sequence variation in the putative effector gene SIX8 facilitates molecular differentiation of Fusarium oxysporum f. sp. cubense. Plant Pathology . n/a-n/a. ISSN 1365-3059

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Article Link(s): http://dx.doi.org/10.1111/ppa.12184

Abstract

Fusarium oxysporum f. sp. cubense (Foc), causal agent of fusarium wilt of banana, is among the most destructive pathogens of banana and plantain. The development of a molecular diagnostic capable of reliably distinguishing between the various races of the pathogen is of key importance to disease management. However, attempts to distinguish isolates using the standard molecular loci typically used for fungal phylogenetics have been complicated by a poor correlation between phylogeny and pathogenicity. Among the available alternative loci are several putative effector genes, known as SIX genes, which have been successfully used to differentiate the three races of F. oxysporum f. sp. lycopersici. In this study, an international collection of Foc isolates was screened for the presence of the putative effector SIX8. Using a PCR and sequencing approach, variation in Foc-SIX8 was identified which allowed race 4 to be differentiated from race 1 and 2 isolates, and tropical and subtropical race 4 isolates to be distinguished from one another.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:avirulence effector Fusarium oxysporum f. sp. cubense molecular diagnostic pathogenicity SIX
Subjects:Plant culture > Fruit and fruit culture
Plant pests and diseases
Deposited On:01 Jul 2014 05:28
Last Modified:01 Jul 2014 05:28

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