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Genetic Structure of Cochliobolus sativus Populations Sampled from Roots and Leaves of Barley and Wheat in North Dakota

Gyawali, S. and Neate, S. M. and Adhikari, T. B. and Puri, K. D. and Burlakoti, R. R. and Zhong, S. B. (2012) Genetic Structure of Cochliobolus sativus Populations Sampled from Roots and Leaves of Barley and Wheat in North Dakota. Journal of Phytopathology, 160 (11-12). pp. 637-646. ISSN 0931-1785

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Article Link(s): http://dx.doi.org/10.1111/j.1439-0434.2012.01956.x

Abstract

Common root rot (CRR) and spot blotch, caused by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dast., are important diseases of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) worldwide. However, the population biology of C. sativus is still poorly understood. In this study, the genetic structure of three C. sativus populations, consisting of isolates sampled respectively from barley leaves (BL), barley roots (BR) and wheat roots (WR) in North Dakota, was analysed with amplified fragment length polymorphism (AFLP) markers. A total of 127 AFLP loci were generated among 208 C. sativus isolates analysed with three primer combinations. Gene diversity (H = 0.277-0.335) were high in all three populations. Genetic variation among C. sativus individuals within population accounted for 74%, whereas 26% of the genetic variation was explained among populations. Genetic differentiation was high (empty set PT = 0.261, corrected G ''(st)= 0.39), whereas gene flow (Nm) ranged from 1.27 to 1.56 among the three populations analysed. The multilocus linkage disequilibrium (LD) ((r) over bard = 0.0760.117) was moderate in C. sativus populations. Cluster analyses indicate that C. sativus populations differentiated according to the hosts (barley and wheat) and tissues (root and leaf) although generalists also exist in North Dakota. Crop breeding may benefit from combining genes for resistance against both specialists and generalists of C. sativus.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Gyawali, Sanjaya Neate, Stephen M. Adhikari, Tika B. Puri, Krishna D. Burlakoti, Rishi R. Zhong, Shaobin
Subjects:Plant pests and diseases > Individual or types of plants or trees > Wheat
Plant pests and diseases > Individual or types of plants or trees > Barley
Plant culture > Field crops
Deposited On:09 Apr 2014 21:08
Last Modified:11 Apr 2014 15:08

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