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Determining changes in the distribution and abundance of a Rhyzopertha dominica phosphine resistance allele in farm grain storages using a DNA marker

Kaur, R. and Daniels, E. V. and Nayak, M. K. and Ebert, P. R. and Schlipalius, D. I. (2013) Determining changes in the distribution and abundance of a Rhyzopertha dominica phosphine resistance allele in farm grain storages using a DNA marker. Pest Management Science, 69 (6). pp. 685-688. ISSN 1526498X (ISSN)

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Article Link(s): http://dx.doi.org/10.1002/ps.3514

Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/ps.3514/abstract

Abstract

BACKGROUND: The lesser grain borer, Rhyzopertha dominica (F.), is a highly destructive pest of stored grain that is strongly resistant to the fumigant phosphine (PH3). Phosphine resistance is due to genetic variants at the rph2 locus that alter the function of the dihydrolipoamide dehydrogenase (DLD) gene. This discovery now enables direct detection of resistance variants at the rph2 locus in field populations. RESULTS: A genotype assay was developed for direct detection of changes in distribution and frequency of a phosphine resistance allele in field populations of R. dominica. Beetles were collected from ten farms in south-east Queensland in 2006 and resampled in 2011. Resistance allele frequency increased in the period from 2006 to 2011 on organic farms with no history of phosphine use, implying that migration of phosphine-resistant R. dominica had occurred from nearby storages. CONCLUSION: Increasing resistance allele frequencies on organic farms suggest local movement of beetles and dispersal of insects from areas where phosphine has been used. This research also highlighted for the first time the utility of a genetic DNA marker in accurate and rapid determination of the distribution of phosphine-resistant insects in the grain value chain. Extending this research over larger landscapes would help in identifying resistance problems and enable timely pest management decisions. © 2013 Society of Chemical Industry © 2013 Society of Chemical Industry 69 6 June 2013 10.1002/ps.3514 Rapid Report Rapid Report © 2013 Society of Chemical Industry.

Item Type:Article
Business groups:Crop and Food Science
Keywords:Marker Resistance gene Resistance monitoring Rhyzopertha dominica abundance allele beetle chemical compound food storage fumigant genetic marker pest control pest species pesticide resistance population distribution Australia Queensland Coleoptera Hexapoda
Subjects:Plant culture > Field crops > Grain. Cereals
Plant pests and diseases
Deposited On:17 Sep 2013 05:12
Last Modified:17 Sep 2013 05:12

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