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Current and emerging screening methods to identify post-head-emergence frost adaptation in wheat and barley

Frederiks, T. M. and Christopher, J. T. and Harvey, G. L. and Sutherland, M. W. and Borrell, A. K. (2012) Current and emerging screening methods to identify post-head-emergence frost adaptation in wheat and barley. Journal of Experimental Botany, 63 (15). pp. 5405-5416. ISSN 0022-0957

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Article Link(s): http://dx.doi.org/10.1093/jxb/ers215

Abstract

Cereal crops can suffer substantial damage if frosts occur at heading. Identification of post-head-emergence frost (PHEF) resistance in cereals poses a number of unique and difficult challenges. Many decades of research have failed to identify genotypes with PHEF resistance that could offer economically significant benefit to growers. Research and breeding gains have been limited by the available screening systems. Using traditional frost screening systems, genotypes that escape frost injury in trials due to spatial temperature differences and/or small differences in phenology can be misidentified as resistant. We believe that by improving techniques to minimize frost escapes, such ofalse-positive' results can be confidently identified and eliminated. Artificial freezing chambers or manipulated natural frost treatments offer many potential advantages but are not yet at the stage where they can be reliably used for frost screening in breeding programmes. Here we describe the development of a novel photoperiod gradient method (PGM) that facilitates screening of genotypes of different phenology under natural field frosts at matched developmental stages. By identifying frost escapes and increasing the efficiency of field screening, the PGM ensures that research effort can be focused on finding genotypes with improved PHEF resistance. To maximize the likelihood of identifying PHEF resistance, we propose that the PGM form part of an integrated strategy to (i) source germplasm;(ii) facilitate high throughput screening; and (iii) permit detailed validation. PGM may also be useful in other studies where either a range of developmental stages and/or synchronized development are desired.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Frederiks, T. M. Christopher, J. T. Harvey, G. L. Sutherland, M. W. Borrell, A. K. Queensland Government; Australian Grains Research and Development Corporation (GRDC) [DAQ472, DAQ00002, UQ00052] Thanks to D. Woodruff (retired) and W. Single (retired) for generously providing advice, encouragement, published papers, and unpublished data. Statisticians D. Butler, A. Kelly, D. Burrell, and S. Fletcher provided trial design layouts, analysis, and advice. Field staff at the Leslie Research Facility and Hermitage Research Facility, including J. Henderson, G. Pauli, G. Kedzlie, and D. Browne, are thanked, as well as R. Ferguson and R. Williams for their critical evaluation and assistance in the preparation of this manuscript. This work was supported by the Queensland Government and the Australian Grains Research and Development Corporation (GRDC) [DAQ472, DAQ00002, UQ00052]. Oxford univ press Oxford
Keywords:Barley in-head frost reproductive frost spring radiant frost wheat low-temperature tolerance cold-hardiness radiation frost growth-stages winter-wheat injury vernalization expression cereals tests
Subjects:Plant culture > Field crops > Grain. Cereals
Science > Botany > Genetics
Deposited On:15 Oct 2013 05:02
Last Modified:15 Oct 2013 05:02

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