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Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato

Walmsley, A.M. and Alvarez, M.L. and Jin, Y. and Kirk, D.D. and Lee, S.M. and Pinkhasov, J. and Rigano, M.M. and Arntzen, C.J. and Mason, H.S. (2003) Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato. Plant Cell Reports, 21 (10). pp. 1020-1029.

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Article Link(s): http://dx.doi.org/10.1007/s00299-003-0619-4

Publisher URL: http://www.springerlink.com

Abstract

Epitopes often require co-delivery with an adjuvant or targeting protein to enable recognition by the immune system. This paper reports the ability of transgenic tomato plants to express a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin (LTB) and an immunocontraceptive epitope. The fusion protein was found to assemble into pentamers, as evidenced by its ability to bind to gangliosides, and had an average expression level of 37.8 µg g -1 in freeze-dried transgenic tissues. Processing of selected transgenic fruit resulted in a 16-fold increase in concentration of the antigen with minimal loss in detectable antigen.

The species-specific nature of this epitope was shown by the inability of antibodies raised against non-target species to detect the LTB fusion protein. The immunocontraceptive ability of this vaccine will be tested in future pilot mice studies.

Item Type:Article
Additional Information:© Springer-Verlag GmbH.
Keywords:Heat labile toxin; fusion protein; immunocontraceptive; tomato; transgenic plants.
Subjects:Science > Science (General)
Plant culture > Vegetables
Science > Biology > Genetics
Deposited On:07 May 2004
Last Modified:21 Apr 2011 06:14

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