Dry, I.B. and Yuana, K.H. and Hutton, D.G. (2004) Dicarboximide resistance in field isolates of Alternaria alternata is mediated by a mutation in a two-component histidine kinase gene. Fungal Genetics and Biology, 41 (1). pp. 102-108.
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Article Link(s): http://dx.doi.org/10.1016/j.fgb.2003.09.002
Publisher URL: http://www.elsevier.com
Isolates of Alternaria alternata collected from a field site which had previously been treated with the dicarboximide fungicide iprodione were found to demonstrate a high level of resistance to iprodione and the phenylpyrrole fungicide, fludioxonil in plate assays. In order to determine the genetic basis for this fungicide resistance a partial length clone of a two-component histidine kinase (HK) was isolated from genomic DNA of a fungicide-sensitive A. alternata isolate using degenerate primers by PCR. Analysis of the AaHK1 gene structure indicates the presence of six 90 amino acid repeat domains upstream of a kinase domain as found in the homologous HK genes from other fungal species. Comparison of nucleic acid sequences from the fungicide-sensitive and fungicide-resistant A. alternata isolates confirmed the presence of mutations leading to premature termination of the translated HK protein. The possible role of the two-component HK in the development of dicarboximide resistance in A. alternata is discussed.
|Corporate Creators:||Horticulture and Forestry Science|
|Additional Information:||© Elsevier.|
|Keywords:||Alternaria alternata; dicarboximide; histidine kinase; fungicide resistance.|
|Subjects:||Science > Botany > Cryptogams|
Science > Biology > Genetics
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Pesticides
|Deposited On:||07 May 2004|
|Last Modified:||31 Mar 2011 00:53|
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