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A monoclonal antibody-blocking Enzyme-linked immunosorbenta assay for the detection of serovar-specific antibodies to haemophilus paragallinarum

Zhang, Z. and Blackall, P.J. and Yamaguchi, T. and Iritani, Y. (1999) A monoclonal antibody-blocking Enzyme-linked immunosorbenta assay for the detection of serovar-specific antibodies to haemophilus paragallinarum. Avian Diseases, 43 (1). pp. 75-82.

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Publisher URL: http://www.aaap.info/mc/page.do

Abstract

Two monoclonal antibody-blocking enzyme-linked immunosorbent assays (B-ELISAs) were developed to detect serovar-specif antibodies to Haemophilus paragallinarum. One assay detected antibodies against serovar A and the other antibodies against serovar C. The assays were evaluated with sera derived from disease-free chickens as well as chickens experimentally immunized and/or challenged with H. paragallinarum strains 0083 (serovar A), Modesto (serovar C), or HP31 (serovar C). When tested with 440 negative sera (170 from a specific-pathogen-free and 30 from each of nine commercial layer flocks), both tests gave only a single false-positive reaction. The use of the B-ELISAs with the experimentally produced sera showed the assays to be serovar specific. With the exception of one serum, the serovar A B-ELISA detected antibodies only in the chickens vaccinated with 0083. Similarly, with the exception of one serum, the serovar C B-ELISA detected antibodies only in those chickens vaccinated with Modesto or those chickens challenged with HP31. Overall, the serovar A B-ELISA had a specificity of 99.7% and a sensivity of 78.7%, whereas the serovar C B-ELISA had a specificity of 99.8% and a sensitivity of 64.7%.

Item Type:Article
Corporate Creators:Animal Science
Additional Information:© American Association of Avian Pathologists.
Keywords:ELISA; Haemophilus paragallinarum; serology; infectious coryza.
Subjects:Science > Science (General)
Veterinary medicine > Diseases of special classes of animals > Avian
Veterinary medicine > Veterinary bacteriology
Deposited On:16 Apr 2004
Last Modified:20 Apr 2011 22:26

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