Zhang, Z. and Blackall, P.J. and Yamaguchi, T. and Iritani, Y. (1999) A monoclonal antibody-blocking Enzyme-linked immunosorbenta assay for the detection of serovar-specific antibodies to haemophilus paragallinarum. Avian Diseases, 43 (1). pp. 75-82.
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Two monoclonal antibody-blocking enzyme-linked immunosorbent assays (B-ELISAs) were developed to detect serovar-specif antibodies to Haemophilus paragallinarum. One assay detected antibodies against serovar A and the other antibodies against serovar C. The assays were evaluated with sera derived from disease-free chickens as well as chickens experimentally immunized and/or challenged with H. paragallinarum strains 0083 (serovar A), Modesto (serovar C), or HP31 (serovar C). When tested with 440 negative sera (170 from a specific-pathogen-free and 30 from each of nine commercial layer flocks), both tests gave only a single false-positive reaction. The use of the B-ELISAs with the experimentally produced sera showed the assays to be serovar specific. With the exception of one serum, the serovar A B-ELISA detected antibodies only in the chickens vaccinated with 0083. Similarly, with the exception of one serum, the serovar C B-ELISA detected antibodies only in those chickens vaccinated with Modesto or those chickens challenged with HP31. Overall, the serovar A B-ELISA had a specificity of 99.7% and a sensivity of 78.7%, whereas the serovar C B-ELISA had a specificity of 99.8% and a sensitivity of 64.7%.
|Corporate Creators:||Animal Science|
|Additional Information:||© American Association of Avian Pathologists.|
|Keywords:||ELISA; Haemophilus paragallinarum; serology; infectious coryza.|
|Subjects:||Science > Science (General)|
Veterinary medicine > Diseases of special classes of animals > Avian
Veterinary medicine > Veterinary bacteriology
|Deposited On:||16 Apr 2004|
|Last Modified:||20 Apr 2011 22:26|
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