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DNA markers linked to the R(2) rust resistance gene in sunflower (Helianthus annuus L.) facilitate anticipatory breeding for this disease variant.

Lawson, W.R. and Jan, C.C and Shattle, L. and Smith, L. and Kong, G.A. and Kochman, J.K. (2012) DNA markers linked to the R(2) rust resistance gene in sunflower (Helianthus annuus L.) facilitate anticipatory breeding for this disease variant. Molecular Breeding, 28 (4). pp. 569-579.

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Article Link(s): http://dx.doi.org/10.1007/s11032-010-9506-1

Publisher URL: http://www.springeronline.com

Abstract

Pre-emptive breeding for host disease resistance is an effective strategy for combating and managing devastating incursions of plant pathogens. Comprehensive, long-term studies have revealed that virulence to the R (2) sunflower (Helianthus annuus L.) rust resistance gene in the line MC29 does not exist in the Australian rust (Puccinia helianthi) population. We report in this study the identification of molecular markers linked to this gene. The three simple sequence repeat (SSR) markers ORS795, ORS882, and ORS938 were linked in coupling to the gene, while the SSR marker ORS333 was linked in repulsion. Reliable selection for homozygous-resistant individuals was efficient when the three markers, ORS795, ORS882, and ORS333, were used in combination. Phenotyping for this resistance gene is not possible in Australia without introducing a quarantinable race of the pathogen. Therefore, the availability of reliable and heritable DNA-based markers will enable the efficient deployment of this gene, permitting a more effective strategy for generating sustainable commercial cultivars containing this rust resistance gene.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science
Keywords:Helianthus annuus L.; sunflower; rust resistance; pre-emptive breeding; SSR markers; assisted selection; puccinia-helianthi; arbitrary primers; coupling-phase; map; polymorphisms; inheritance; diversity; Australia.
Subjects:Science > Biology > Molecular Biology
Plant culture
Science > Biology > Genetics
Deposited On:20 Feb 2012 07:54
Last Modified:18 Sep 2013 03:29

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