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Application of ERIC–PCR for the comparison of isolates of Haemophilus parasuis

Rafiee, M. and Bara, M. and Stephens, C.P. and Blackall, P.J. (2000) Application of ERIC–PCR for the comparison of isolates of Haemophilus parasuis. Australian Veterinary Journal, 78 (12). pp. 846-849.

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Article Link(s): http://dx.doi.org/10.1111/j.1751-0813.2000.tb10507...

Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home

Abstract

Objectives:
To produce antisera to the 15 recognised reference strains of the Kielstein-Rapp Gabrielson (KRG) serotyping scheme for Haemophilus parasuis, validate those sera and use them to serotype 46 Australian field isolates of H parasuis.

Design:
Antisera were produced in rabbits and validated by cross-testing with the reference strains and re-testing 15 Australian field isolates of H parasuis that had been previously serotyped in the United States of America. The validated antisera were then used to determine the serovar of 46 Australian isolates.

Results:
Monospecific antisera were produced for 14 of the 15 KRG serovars of H parasuis. Two Australian field isolates, confirmed previously as serovars 1 and 7, were used to produce monospecific antisera for serovars 1 and 7 respectively. The antiserum for serovar 4 gave a one-way cross reaction with the antigen of serovar 14. The typing antisera correctly typed all 15 H parasuis that had been previously typed by antisera produced overseas. The 46 field isolates were shown to belong to serovars 2 (two isolates), 4 (one isolate), 5 (18 isolates), 12 (two isolates) and 13 (four isolates). The remaining 19 isolates were non-typable.

Conclusion:
Serotyping of H parasuis isolates is now available in Australia. H parasuis serovars 5 and 13 remain the predominant serovars present in Australian pigs.

Item Type:Article
Additional Information:Reproduced with permission from the © Australian Veterinary Journal. Access to published version may be available via Publisher’s website.
Keywords:Haemophilus parasuis; serological characterisation; KRG scheme.
Subjects:Veterinary medicine > Veterinary bacteriology
Deposited On:26 Mar 2004
Last Modified:10 Sep 2010 02:55

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