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Characterisation of a novel Mannheimia sp from Australian feedlot cattle

Blackall, P.J. and Angen, O. and Fegan, N. and Blackall, L.L. and Mutters, R. and Bisgaard, M. (2001) Characterisation of a novel Mannheimia sp from Australian feedlot cattle. Australian Veterinary Journal, 79 (9). pp. 634-639.

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Article Link(s): http://dx.doi.org/10.1111/j.1751-0813.2001.tb10787...

Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home

Abstract

Objective: To characterise eight isolates of a Gram-negative organism obtained from the upper respiratory tract of cattle showing evidence of mild upper respiratory tract disease.
Design: The isolates were compared with the five recognised species within the genus Mannheimia – M haemolytica, M glucosida, M granulomatis, M ruminalisand M varigena- using a range of phenotypic and genotypic methods.
Results: Phenotypic characterisation indicated that the isolates belonged to the trehalose-negative [Pasteurella] haemolytica complex. This complex has recently been reorganized into five species within the new genus Mannheimia. Ribotyping performed using HindIII and a computerized analysis system indicated that the eight Australian isolates formed a distinct cluster that was related to, but different from, the five recognised species of Mannheimia. The 16S rRNA sequence of one isolate (BNO311) was determined and a phylogenetic analysis performed. Isolate BNO311 was distinct from the five named Mannheimia spp but did join a larger cluster consisting of rRNA cluster IV (M varigena) and the unnamed rRNA cluster V of Mannheimia. DNA:DNA hybridisation between isolate BNO311 and M haemolytica NCTC 9380T, M granulomatisP411 and Actinobacillus ligniersii NCTC 4189T all suggested similarities of approximately 30%.
Conclusions: These phenotypic and genotypic characterisation studies suggest that the eight Australian isolates represent a new species of Mannheimia. Until further characterisation studies are performed, we are unwilling to propose a name for this taxon, preferring to refer to this possible new species as Bisgaard taxon 39 of cluster V of Mannheimia.

Item Type:Article
Corporate Creators:Animal Science
Additional Information:Reproduced with permission from the © Australian Veterinary Journal. Access to published version may be available via Publisher’s website.
Keywords:Mannheimia haemolytica; Mannheimia glucosida; Mannheimia granulomatis; Mannheimia ruminalis; Mannheimia varigena; Characterisation; Pasteurella.
Subjects:Veterinary medicine > Other diseases and conditions
Veterinary medicine > Communicable diseases of animals (General)
Animal culture > Cattle
Deposited On:24 Aug 2004
Last Modified:13 Aug 2010 15:08

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