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Molecular analysis of a haemagglutinin of Haemophilus paragallinarum

Hobb, R.I. and Tseng, H.J. and Downes, J.E. and Terry, T.D. and Blackall, P.J. and Takagi, M. and Jennings, M.P. (2002) Molecular analysis of a haemagglutinin of Haemophilus paragallinarum. Microbiology, 148 (7). pp. 2171-2179.

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Publisher URL: http://www.sgmjournals.org/

Abstract

The gene encoding a haemagglutinin of H. paragallinarum, hagA, has been identified and the full-length nucleotide sequence determined. A 39 kDa protein, recognized by an anti-haemagglutinin monoclonal antibody, mAb4D, was purified from H. paragallinarum strain 0083 and the N-terminal sequence obtained. The full-length nucleotide sequence was obtained by inverse PCR and the deduced amino acid sequence of the protein encoded was shown to be similar to other outer-membrane proteins of closely related organisms in the HAP group (Haemophilus, Actinobacillus, Pasteurella), especially the P5 protein of Haemophilus influenzae. The hagA gene was cloned into a His-tag expression vector and overexpressed in Escherichia coli strain M15(pREP4). The identity of the purified recombinant protein as a H. paragallinarum haemagglutinin was confirmed by haemagglutination of chicken red blood cells and reactivity, in a Western blot, with the monoclonal antibody specific for the serovar A haemagglutinin.

Item Type:Article
Additional Information:Reproduced with permission from the Society of General Microbiology. Access to published version may be available via Publisher’s website.
Keywords:Serotyping antigen; infectious coryza; outer-membrane protein.
Subjects:Veterinary medicine > Diseases of special classes of animals > Avian
Science > Biology > Genetics
Veterinary medicine > Veterinary bacteriology
Deposited On:26 Mar 2004
Last Modified:26 Nov 2010 03:53

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