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Evaluation of four serological tests for the diagnosis of caprine melioidosis

Thomas, A. D. and Spinks, G. A. and D'Arcy, T. L. and Norton, J. H. and Trueman, K. F. (1988) Evaluation of four serological tests for the diagnosis of caprine melioidosis. Australian Veterinary Journal, 65 (9). pp. 261-264.

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Abstract

A complement fixation (CF) test, two indirect haemagglutination (IHA-A; IHA-L) tests which differed in antigen preparation and technique, and a microtitre agglutination (MA) test were compared in the serodiagnosis of melioidosis in goats. 118 experimental serum samples and 3143 field serum samples from goats in endemic and non-endemic areas of north Queensland were used. Culture of samples for Pseudomonas pseudomallei from 112 goats provided substantiating evidence of infection.
The IHA-A test was the most sensitive, and the CF test the most specific. It is recommended that the IHA-A be used as a screening test followed by the CF test for confirmation of active melioidosis. The IHA-A test is the better indicator of past infection. The four tests were compared using 577 serum samples from goats in areas where melioidosis is endemic; 42 were positive, 34 by IHA-A, 33 by MA and 30 by CFT.
Further tests are described using serum samples from 2480 goats grazing in endemic and non-endemic areas.

Item Type:Article
Additional Information:Reproduced with permission from the © Australian Veterinary Journal. Access to published version may be available via Publisher’s website.
Keywords:Goat diseases; bacterial diseases; experimental infection; complement fixation tests; haemagglutination tests; agglutination tests; diagnosis; goats; Burkholderia pseudomallei; Australia; Pseudomonas pseudomallei; Capra; Bovidae; ruminant; Artiodactyla; Mammals; Vertebrates; Chordata; animals; Ungulates; Burkholderia; Burkholderiaceae; Gracilicutes; bacteria; prokaryotes; Australasia; Oceania; Developed Countries; Commonwealth of Nations; OECD Countries; Pseudomonadaceae.
Subjects:Veterinary medicine > Diseases of special classes of animals > Sheep and goats
Veterinary medicine > Veterinary bacteriology
Deposited On:26 Mar 2004
Last Modified:26 Nov 2010 02:28

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