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A new culture method for lesser mealworm, Alphitobius diaperinus

Rice, S.J. and Lambkin, T.A. (2009) A new culture method for lesser mealworm, Alphitobius diaperinus. Journal of Applied Entomology, 133 (1). pp. 67-72.

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Article Link(s): http://dx.doi.org/10.1111/j.1439-0418.2008.01314.x

Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home

Abstract

A new culture method for lesser mealworm, Alphitobius diaperinus (Panzer), was developed to provide large numbers of adult lesser mealworms of approximately the same age for insecticide resistance testing. Culturing entailed allowing 100 adults to reproduce for 4 days in a wheat-based culture medium contained inside a plastic culture box, removing the adults from the medium, and then rearing their progeny to adulthood therein, in approximately 56 days at 32 degrees C and 55% RH. During their development, progeny were supplied water via apple slices at 0, 21 and 35 days, and a foam substrate in which to pupate, also at 35 days. During 2004-2005, adult lesser mealworms were collected from six broiler-house populations and then cultured with this method. Each population produced 4500 adults required to complete resistance testing with one insecticide within ten culture boxes, at an average of 798 adults per culture box.

Item Type:Article
Corporate Creators:Animal Science
Additional Information:© The Authors. © Blackwell Verlag.
Keywords:Broiler house; darkling beetle; insect culture; litter; mass rearing; baseline responses; reservoir competence; Salmonella typhimurium; Escherichia coli; feeding behavior; fed larvae; Tenebrionidae; Coleoptera; Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae).
Subjects:Animal culture > Insect culture and beneficial insects > Other insects
Animal culture > Housing and environmental control
Science > Biology > Reproduction
Animal culture > Feeds and feeding. Animal nutrition
Deposited On:14 Apr 2009 06:51
Last Modified:12 Apr 2011 22:05

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