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Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses

Gambley, C.F. and Geering, A.D.W. and Thomas, J.E. (2009) Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses. Journal of Virological Methods, 155 (2). pp. 187-192.

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Article Link(s): http://dx.doi.org/10.1016/j.jviromet.2008.10.006

Publisher URL: http://www.elsevier.com

Abstract

A semi-automated, immunomagneticcapture-reverse transcription PCR(IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science
Additional Information:© Crown Copyright.
Keywords:Semi-automation; mealybug wilt disease; pineapple mealybug wilt-associated virus; RT-PCR; Hepatitis A virus; polymerase chain reaction (PCR); hybridization RT-PCR; genetic diversity; immunocapture; Australia; disease; banana.
Subjects:Science > Science (General)
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Plant pests and diseases > Plant pathology
Science > Biology > Genetics
Deposited On:16 Apr 2009 02:45
Last Modified:20 Oct 2011 06:39

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