Login | Create Account (DAF staff only)

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Garland, S. and Sharman, M. and Persley, D. and McGrath, D. (2005) The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Australian Journal of Agricultural Research, 56 (3). pp. 285-289.

[img] PDF
171kB

Article Link(s): http://dx.doi.org/10.1071/AR04140

Publisher URL: http://www.publish.csiro.au/

Abstract

Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science, Horticulture and Forestry Science
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Rapid DNA extraction; disease resistance; genetic marker; polymerase chain reaction; viral disease; Lycopersicon esculentum; Tomato spotted wilt virus; Tospovirus.
Subjects:Plant pests and diseases > Individual or types of plants or trees
Plant culture > Fruit and fruit culture
Science > Biology > Genetics
Deposited On:27 Feb 2009 05:32
Last Modified:24 Oct 2011 01:11

Repository Staff Only: item control page