Garland, S. and Sharman, M. and Persley, D. and McGrath, D. (2005) The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Australian Journal of Agricultural Research, 56 (3). pp. 285-289.
Article Link(s): http://dx.doi.org/10.1071/AR04140
Publisher URL: http://www.publish.csiro.au/
Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.
|Business groups:||Agri-Science, Crop and Food Science, Horticulture and Forestry Science|
|Additional Information:||Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.|
|Keywords:||Rapid DNA extraction; disease resistance; genetic marker; polymerase chain reaction; viral disease; Lycopersicon esculentum; Tomato spotted wilt virus; Tospovirus.|
|Subjects:||Plant pests and diseases > Individual or types of plants or trees|
Plant culture > Fruit and fruit culture
Science > Biology > Genetics
|Deposited On:||27 Feb 2009 05:32|
|Last Modified:||24 Oct 2011 01:11|
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