Login | Create Account (DAF staff only)

Use of optical density as a measure of Claviceps africana conidial suspension concentration

Herde, D.J. and Ryley, M.J. and Foster, S.D. and Galea, V.J. and Henzell, R.G. and Jordan, D.R. (2006) Use of optical density as a measure of Claviceps africana conidial suspension concentration. Australasian Plant Pathology, 35 (1). pp. 77-80.

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://dx.doi.org/10.1071/AP05093

Publisher URL: http://www.publish.csiro.au/

Abstract

Sorghum ergot, caused by Claviceps africana, has remained a major disease problem in Australia since it was first recorded in 1996, and is the focus of a range of biological and integrated management research. Artificial inoculation using conidial suspensions is an important tool in this research. Ergot infection is greatly influenced by environmental factors, so it is important to reduce controllable sources of variation such as inoculum concentration. The use of optical density was tested as a method of quantifying conidial suspensions of C. africana, as an alternative to haemocytometer counts. This method was found to be accurate and time efficient, with possible applications in other disease systems.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science
Additional Information:© Australasian Plant Pathology Society. © CSIRO Publishing.
Keywords:Colorimeter; Inoculum concentration; light transmittance; Sorghum bicolor; sorghum; ergot; disease.
Subjects:Technology > Technology (General)
Science > Statistics
Plant culture > Field crops > Grain. Cereals
Plant pests and diseases
Deposited On:12 Feb 2009 05:24
Last Modified:25 Oct 2011 03:43

Repository Staff Only: item control page