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Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area

Molad, T. and Mazuz, M.L. and Fleiderovitz, L. and Fish, L. and Savitsky, I. and Krigel, Y. and Leibovitz, B. and Shkap, V. and Molloy, J. and Jongejan, F. (2006) Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area. Veterinary Microbiology, 113 (1-2). pp. 55-62.

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Article Link(s): http://dx.doi.org/10.1016/j.vetmic.2005.10.026

Publisher URL: http://www.elsevier.com

Abstract

A reverse line blot hybridization (RLB) one-stage nested PCR (nPCR) for Anaplasma centrale and a nested PCR for Anaplasma marginale were used to detect infected cattle grazing within an endemic region in Israel. A novel set of PCR primers and oligonucleotide probes based on a 16S ribosomal RNA gene was designed for RLB detection of both Anaplasma species, and the performance of the molecular assays compared. The immunofluorescent antibody test (IFA) was used to detect antibodies to both Anaplasma species, whereas, a highly sensitive and specific competitive enzyme-linked immunosorbent assay (cELISA) was used to detect antibodies in A. centrale-vaccinated cattle. The RLB and the nested PCR procedures showed bacteremia with sensitivity of 50 infected erythrocytes per milliliter. Up to 93% of the A. centrale vaccinates carried specific antibodies that were detected by cELISA, and up to 71% of the vaccinated cattle were found to be naturally infected with A. marginale according to the PCR and the RLB assays. Nevertheless, no severe outbreaks of A. marginale infection occurred among vaccinated herds in this endemic region. It appears that both, molecular tools and serology are useful for evaluation of the vaccine efficacy. In the light of wide natural field infection with A. marginale, strong recommendations to continue the A. centrale vaccination program regime will continue until a new generation of non-blood-based vaccine will be developed.

Item Type:Article
Additional Information:© Elsevier B.V.
Keywords:Anaplasma; reverse blot line hybridization; serology; vaccination.
Subjects:Science > Science (General)
Veterinary medicine > Diseases of special classes of animals > Cattle
Veterinary medicine > Veterinary bacteriology
Deposited On:24 Feb 2009 05:33
Last Modified:10 Sep 2010 00:23

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