Login | Create Account (DAF staff only)

A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus)

McHale, M. and Broderick, D. and Ovenden, J.R. and Lanyon, J.M. (2008) A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). Molecular Ecology Resources, 8 (3). pp. 669-670.

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://dx.doi.org/10.1111/j.1471-8286.2007.02041.x

Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home

Abstract

Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.

Item Type:Article
Additional Information:© The State of Queensland Through its Department of Primary Industries and Fisheries.
Keywords:Dugong; manatee; molecular sexing; SRY; ZFX; ZFY.
Subjects:Aquaculture and Fisheries > Fisheries > Fishery research
Science > Biology > Genetics
Science > Zoology > Chordates. Vertebrates > Mammals
Deposited On:02 Feb 2009 04:23
Last Modified:07 Sep 2010 07:26

Repository Staff Only: item control page