McHale, M. and Broderick, D. and Ovenden, J.R. and Lanyon, J.M. (2008) A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). Molecular Ecology Resources, 8 (3). pp. 669-670.
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Article Link(s): http://dx.doi.org/10.1111/j.1471-8286.2007.02041.x
Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home
Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.
|Additional Information:||© The State of Queensland Through its Department of Primary Industries and Fisheries.|
|Keywords:||Dugong; manatee; molecular sexing; SRY; ZFX; ZFY.|
|Subjects:||Aquaculture and Fisheries > Fisheries > Fishery research|
Science > Biology > Genetics
Science > Zoology > Chordates. Vertebrates > Mammals
|Deposited On:||02 Feb 2009 04:23|
|Last Modified:||07 Sep 2010 07:26|
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