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Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza

Corney, B.G. and Diallo, I.S. and Wright, L. and Hewitson, G. and De Jong, A. and Tolosa, X. and Burrell, P. and Duffy, P. and Rodwell, B. and Boyle, D.B. and Blackall, P.J. (2008) Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5' Taq nuclease assay: A new tool for diagnosing infectious coryza. Avian Pathology, 37 (6). pp. 599-604.

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Article Link(s): http://dx.doi.org/10.1080/03079450802449139

Publisher URL: http://www.tandf.co.uk/journals/alphalist.asp

Abstract

A 5' Taq nuclease assay specific for Avibacterium paragallinarum was designed and optimized for use in diagnosing infectious coryza. The region chosen for assay design was one of known specificity for Av. paragallinarum. The assay detected Av. paragallinarum reference strains representing the three Page and the eight Kume serovars, and field isolates from diverse geographical locations. No cross-reactions were observed with other Avibacterium species, with other bacteria taxonomically related to Av. paragallinarum nor with bacteria and viruses likely to be present in swabs collected from suspected infectious coryza cases. The detection limit for the assay was 6 to 60 colony-forming units per reaction. Twenty-two out of 53 swabs collected from sick birds reacted in the 5' Taq nuclease assay, whereas Av. paragallinarum was not isolated from any of the swabs. All of the 22 swabs yielded other bacteria in culture. The presence of Av. paragallinarum in the swabs was also demonstrated by sequencing, thereby confirming the ability of the assay to detect Av. paragallinarum in the presence of other bacteria. The ability to quantify bacterial load in the swabs using the 5' Taq nuclease assay was demonstrated.

Item Type:Article
Corporate Creators:Biosecurity Queensland, Animal Science
Additional Information:© Houghton Trust Ltd.
Keywords:Avibacterium paragallinarum; Coryza; Av. Paragallinarum; Page serovars; Kume serovars; 5' Taq nuclease assay.
Subjects:Veterinary medicine > Diseases of special classes of animals > Avian
Veterinary medicine > Veterinary bacteriology
Deposited On:03 Feb 2009 05:55
Last Modified:08 Sep 2010 05:13

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