Collard, B.C.Y. and Jolley, R. and Bovill, W.D. and Grams, R.A. and Wildermuth, G.B. and Sutherland, M.W. (2006) Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'. Australian Journal of Agricultural Research, 57 (9). pp. 967-973.
Article Link(s): http://dx.doi.org/10.1071/AR05419
Publisher URL: http://www.publish.csiro.au
We have tested the efficacy of putative microsatellite single sequence repeat (SSR) markers, previously identified in a 2-49 (Gluyas Early/Gala) × Janz doubled haploid wheat (Triticum aestivum) population, as being linked to partial seedling resistance to crown rot disease caused by Fusarium pseudograminearum. The quantitative trait loci (QTLs) delineated by these markers have been tested for linkage to resistance in an independent Gluyas Early × Janz doubled haploid population. The presence of a major QTL on chromosome 1DL (QCr.usq-1D1) and a minor QTL on chromosome 2BS (QCr.usq-2B1) was confirmed. However, a putative minor QTL on chromosome 2A was not confirmed. The QTL on 1D was inherited from Gluyas Early, a direct parent of 2-49, whereas the 2B QTL was inherited from Janz. Three other putative QTLs identified in 2-49 × Janz (on 1AL, 4BL, and 7BS) were inherited by 2-49 from Gala and were not able to be confirmed in this study. The screening of SSR markers on a small sample of elite wheat genotypes indicated that not all of the most tightly linked SSR markers flanking the major QTLs on 1D and 1A were polymorphic in all backgrounds, indicating the need for additional flanking markers when backcrossing into some elite pedigrees. Comparison of SSR haplotypes with those of other genotypes exhibiting partial crown rot resistance suggests that additional, novel sources of crown rot resistance are available.
|Business groups:||Agri-Science, Crop and Food Science|
|Additional Information:||Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.|
|Keywords:||1-LOD support interval; allelic homoplasy; QTL resolution; targetted mapping.|
|Subjects:||Plant culture > Seeds. Seed technology|
Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Plant pests and diseases > Individual or types of plants or trees > Wheat
|Deposited On:||02 Feb 2009 00:54|
|Last Modified:||27 Oct 2011 01:22|
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