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Genetic characterisation of bovine herpesvirus 1 in New Zealand

Wang, J. and Horner, G.W. and O'Keefe, J.S. (2006) Genetic characterisation of bovine herpesvirus 1 in New Zealand. New Zealand Veterinary Journal, 54 (2). pp. 61-66.

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Organisation URL: http://www.vetjournal.org.nz/

Abstract

AIM: To genotype bovine herpesvirus type 1 (BHV-1) isolates from cattle in New Zealand.

METHODS: Twenty-eight BHV-1 isolates were collected from clinical samples from cattle over 28 years. They were characterised and compared using restriction endonuclease analysis (REA), and polymerase chain reaction (PCR) and DNA sequencing.

RESULTS: Twenty-four isolates were classified as bovine herpesvirus subtype 1.2b (BHV-1.2b) by REA. The remaining four isolates were distinct from the others in REA profiles of one of the major enzymes (HindIII) by which the classification was made. However, these four isolates were closely related to others when the REA profiles of other restriction enzymes were studied, and therefore were regarded as divergent strains of BHV-1.2b. All BHV-1 isolates were detectable by PCR, and sequence analysis of selected PCR products did not indicate any significant differences between isolates.

CONCLUSION: BHV-1.2b appears to be the predominant strain of BHV-1 in cattle in New Zealand. There was no evidence that more virulent strains of BHV-1, e.g. subtype 1.1 and BHV type 5, are, or have been, present in New Zealand. Genetic variations exist among these BHV-1.2b isolates.

Item Type:Article
Additional Information:© New Zealand Veterinary Association.
Keywords:Bovine herpesvirus type 1 (BHV-1); bovine herpesvirus subtype 1.2b (BHV-1.2b); polymerase chain reaction (PCR); restriction endonuclease analysis (REA).
Subjects:Science > Biology > Genetics
Veterinary medicine > Veterinary virology
Veterinary medicine > Diseases of special classes of animals > Cattle
Deposited On:27 Jan 2009 02:39
Last Modified:23 Jun 2011 03:18

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